Super Micro Bead Silica Gel

FUJI SILYSIA CHEMICAL LTD.

CHROMATOREX®

liquid chromatography stationary phase

Silica gel is the most widely used stationary phase for

Liquid Chromatography.

Its physical and chemical properties make it suitable

for a wide variety of analytical and preparative separations.

It provides outstanding characteristics regarding

selectivity, capacity and efficiency for all types of chromatography.

One application

f

One grade

FUNCTIONALITY

The solubility of the targeted compound to be purified contributes to the

determination of the functionality of the silica.

SELECTIVITY

The resolution factor defines the efficiency (number of theoretical

plates) required to perform the separation, and give an indication about

the particle size to be selected.

CAPACITY

The molecular weight of the targeted compound is leading the choice of

the pore size of the silica to optimize the capacity.

There are 3 areas where the

choice of a stationary phase

for a liquid chromatography

application is crucial.

.more

One Application

Spherical Silica / SMB

Description

CHROMATOREX-SMB, Super Micro Bead spherical

silica gels, manufactured by Fuji Silysia, are ideally

suited for the normal phase chromatography, both

analytical and preparative HPLC.

ALL CHROMATOREX-SMB spherical silica gels are

mechanically robust. Therefore, they can be repacked

multiple times in either analytical or preparative scale

columns. Moreover, each production batch has

undergone extensive quality control testing to ensure

reliable lot-to-lot performance.

The availability of a wide range of pore sizes, with

narrow distribution, permits the utilization for a broad

range of applications allowing the optimization of

selectivity and capacity. Tight particle size distributions

are ideally suited to choose the best compromise

between efficiency, operating pressure and process

time, thus offering the best balance between price and

performance.

CHROMATOREX-SMB spherical silica gels are

manufactured to very high chemical purity standards.

Organics and trace metals content are controlled within

stringent limits. Reproducible separation results,

allowing direct scale-up without time-consuming

methods redevelopment, are the direct result of the

rigid control of chemical purity, surface area, pore

volume, and pore size.

Featuresss & Benefits

◆ Selection of particle size to optimize efficiency.

◆ Narrow particle size distribution to reduce pressure.

◆ Consistent and reproducible retention times to allow

direct scale-up from lab through process applications.

◆ Lot-to-lot consistency for reproducible performance.

◆ Bulk quantities for large-scale applications, no scale-up

limitation, secured lots offered for process use.

Characteristics

SMB70 SMB100 SMB200 SMB300
Nominal Pore Diameter nm 7 10 20 30
Specific Surface Area m2/g 480 270 130 90
Particle Sizes オm 5, 10, 15, 20, 20/45
Specific Pore Volume ml/g 0.8
pH (5% suspension) 5.5
Density g/ml 0.4
Water Content % <2
Chemical Analysis SiO2 % 99.9
Na2O % 0.02
Fe2O3 % 0.01
CaO % 0.02
Al2O3% 0.03

Particle Sizes

For analytical HPLC, CHROMATOREX-SMB offer a

selection between 3 and 5 オm. The 5 オm particles are

particularly recommended for method development

start-up to help the optimization of the method.

For preparative HPLC, either at pilot stage or for high

value purification processes, the 10 オm particles are

preferred for those applications requiring higher

resolution, while the 15 オm particles offer a good

compromise between performance and cost.

The larger particle size 20 and 20 / 45 オm are well

suited for medium pressure columns.

Pore Sizes

CHROMATOREX-SMB with pore sizes 7, 10 and 20

nm are recommended for the purification of natural

and synthetic small molecules, lipid soluble organic,

small organic chemical compounds.

Larger pore sizes, 30, 50, 80 or 100 nm are

preferred to optimize the capacity and the yield for

the purification of medium to large peptides and

proteins.

Spherical Silica / MB

Description

CHROMATOREX-MB, Micro Bead spherical silica

gels, manufactured by Fuji Silysia, are ideally suited

for the normal phase chromatography, flash

chromatography and absorption.

ALL CHROMATOREX-MB spherical silica gels are

mechanically robust. Therefore, they can be repacked

multiple times in either analytical or preparative scale

columns. Moreover, each production batch has

undergone extensive quality control testing to ensure

reliable lot-to-lot performance.

The availability of a wide range of pore sizes, with

narrow distribution, permits the utilization for a broad

range of applications allowing the optimization of

selectivity and capacity. Tight particle size distributions

are ideally suited to choose the best compromise

between efficiency, operating pressure and process

time, thus offering the best balance between price and

performance.

CHROMATOREX-MB spherical silica gels are

manufactured to very high chemical purity standards.

Organics and trace metals content are controlled within

stringent limits. Reproducible separation results,

allowing direct scale-up without time-consuming

methods redevelopment, are the direct result of the

rigid control of chemical purity, surface area, pore

volume, and pore size.

Featuresss & Benefits

◆ Selection of particle size to optimize efficiency.

◆ Spherical particle shape to reduce pressure.

◆ Consistent and reproducible retention times to allow

direct scale-up from laboratory through process

applications.

◆ Lot-to-lot consistency for reproducible performance.

◆ Bulk quantities for large-scale applications, no scale-up

limitation, secured lots offered for process use.

Characteristics

SMB70 SMB100 SMB300
Nominal Pore Diameter nm 7 10 30
Specific Surface Area m2/g 480 270 90
Particle Size Distribution オm 40/75 & 75/200
Specific Pore Volume ml/g 0.8
pH (5% suspension) 7
Density g/ml 0.5
Water Content % 5
Chemical Analysis SiO2 % 99.9
Na2O % 0.02
Fe2O3 % 0.01
CaO % 0.02
Al2O3% 0.03

Particle Sizes

Silica gel quality is critical to achieve optimum

performance when using the Flash chromatography

method.

The particle size distribution and the particle shape

determine the resolution and the speed.

For efficient mass transfer, CHROMATOREX-MB

spherical silica gels have a tight control of particle

size distribution allowing less than 10% particles

upside and downside the defined distribution range.

Two standard particle size distributions are offered.

Pore Sizes

Surface area, water content and surface pH

determine the capacity of the silica gel.

CHROMATOREX-MB is fully porous silica, and the

rigorous control of the pore volume and pore size

distribution ensures the accessibility for the targeted

molecule to reach the maximum surface area.

CHROMATOREX-MB with pore sizes 7 and 10 nm

and recommended for the purification of natural and

synthetic small molecules, lipid soluble organic,

small organic chemical compounds.

Larger pore sizes, 30 nm are preferred to optimize

the capacity and the yield for the purification of

medium to large peptides and proteins.

Granular Silica

Description

CHROMATOREX-GS, granular silica gels,

manufactured by Fuji Silysia, are the most popular

grade for the normal phase adsorption chromatography.

Mechanically robust, CHROMATOREX-GS granular

silica gels are cost effective, and therefore they are

very often used only one time, and not regenerated,

due to the cost of the solvent regeneration versus the

price of the silica.

Fuji Silysia is certainly the sole company, due to its

historical roots, to manufacture the base material for

the production of the silica gels. By doing this, we are

ensuring a high level of consistency and reproducibility.

In addition our large scale production, in multi 1,000

tons, of silica gels per year, give us a significant

advantage in the process control, resulting in

outstanding quality and reliable lot-to-lot performance.

CHROMATOREX-GS granular silica gels are

manufactured to very high chemical purity standards.

Organics and trace metals content are controlled within

stringent limits. Reproducible separation results,

allowing direct scale-up without time-consuming

methods redevelopment, are the direct result of the

rigid control of chemical purity, surface area, pore

volume, and pore size.

Featuresss & Benefits

◆ Selection of particle size to optimize efficiency.

◆ Large surface area to optimize the capacity.

◆ Consistent and reproducible retention times to allow

direct scale-up from laboratory through process

applications.

◆ Lot-to-lot consistency to assure reproducible

performance.

◆ Bulk quantities for large-scale applications, no scale-up

limitation, secured lots offered for process use.

Characteristics

SMB60
Nominal Pore Diameter nm 6
Specific Surface Area m2/g 500
Particle Sizes オm 20/45, 40/75 & 75/200
Specific Pore Volume ml/g 0.75
pH (5% suspension) 6.5
Density g/ml 0.5
Water Content % 5
Chemical Analysis SiO2 % 99.9
Na2O % 0.02
Fe2O3 % 0.01
CaO % 0.02
Al2O3% 0.03

Particle Sizes

CHROMATOREX-GS are particularly well adapted for

the application not requiring a high number of

theoretical plate, such as open column, or low

pressure column, or even batch process.

For efficient mass transfer, CHROMATOREX-GS have

a tight control of particle size distribution allowing less

than 10% particles upside and downside the defined

distribution range.

Three standard particle size distributions are offered:

◆ 30 microns (20/45)

◆ 50 microns (40/75)

◆ 150 microns (75/200)

Special grades for industrial applications are offered in

large bulk quantities:

◆ 30 / 50 microns

◆ 53 / 250 microns

◆ 45 / 100 microns

◆ 75 / 150 microns

◆ 75 / 200 microns

Pore Sizes

CHROMATOREX-GS with 6nm pore size and a

surface area of 500 m2/g is recommended for the

purification of natural and synthetic small molecules,

lipid soluble organic, small organic chemical

compounds.

The large capacity of the CHROMATOREX-GS makes

it widely use for crude pre-purification step.

Bonded Silica

Description

All our silica gels CHROMATOREX can be derivatized

to produce a bonded stationary phase for reverse

phase chromatography or some specific

chromatography types.

Derivatization

The growing interest in complex synthetic or

recombinant peptides has expanded the need for high

performance bonded phase process chromatography.

The use of bonded phases for injectable biological

products has also created new demands.

Optimized quality:

The fact that we manufacture the starting gels in very

large batch quantities constitues an important

contribution to the overall reproducibility of these

reversed phase silica.

Moreover, our production capacity for derivatization

per batch contributes also to a high level of

reproducibility.

Each lot is extensively tasted for carbon load and

chromatography retention times, efficiency and peak

symmetry. The quality of the surface coverage is

tasted by the Phenol/Pyridine test to evaluate any

remaining free acid groups on the silica surface.

Optimized chemistry:

Chromatorex silica gels are manufactured with mono-

functional organo-silanes, and finished with an end

capping surface treatment.

On request, tri-functional, with or without end capping

treatment could be performed.

Each lot is extensively tasted for carbon load and

chromatographic retention times, efficiency and peak

symmetry. The quality of the surface coverage is

tasted by the Phenol/Pyridine test to evaluate any

remaining free acid OH groups on the silica surface.

Product Range

C18 Chromatorex:

This is the most versatile and popular reversed phase.

The choice of the pore size must be carefully selected

in relation to the molecular weight of the molecule.

C8 Chromatorex:

Universal, but less performing than C18, it is the

primary choice for investigation.

Phenyl Chromatorex:

Specific reversed phase for medium / polar molecules,

shows a high selectivity for acceptor / donor

compounds.

C4 Chromatorex:

Extremely performing when bonded in wide pore silica

for bio-chromatography and polar compounds.

NH2 Chromatorex:

The Amino-silica gels will separate a variety of sugars

with acetonitrile / water mobile phase. Alternatively

with hexane / ethyl acetate mobile phase, it will

function as a ‘less active’ normal phase.

In addition, since the surface contains primary amino

groups, NH2 Chromatorex can be used as a base for

coupling chiral ligands.

CN Chromatorex:

Can be used in both normal and reversed phase

chromatographies, for steroids, catechols, …

Diol Chromatorex:

Particularly used for aqueous GPC applications, Diol

Chromatorex could be also used for normal phase

chromatography mode for the separation of phenols for

example.

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