Micro Bead Silica Gel
FUJI SILYSIA CHEMICAL LTD.
CHROMATOREX®
liquid chromatography stationary phase
Silica gel is the most widely used stationary phase for
Liquid Chromatography.
Its physical and chemical properties make it suitable
for a wide variety of analytical and preparative separations.
It provides outstanding characteristics regarding
selectivity, capacity and efficiency for all types of chromatography.
One application
One grade
FUNCTIONALITY
The solubility of the targeted compound to be purified contributes to the
determination of the functionality of the silica.
SELECTIVITY
The resolution factor defines the efficiency (number of theoretical
plates) required to perform the separation, and give an indication about
the particle size to be selected.
CAPACITY
The molecular weight of the targeted compound is leading the choice of
the pore size of the silica to optimize the capacity.
There are 3 areas where the
choice of a stationary phase
for a liquid chromatography
application is crucial.
One Application
Spherical Silica / SMB
Description
CHROMATOREX-SMB, Super Micro Bead spherical
silica gels, manufactured by Fuji Silysia, are ideally
suited for the normal phase chromatography, both
analytical and preparative HPLC.
ALL CHROMATOREX-SMB spherical silica gels are
mechanically robust. Therefore, they can be repacked
multiple times in either analytical or preparative scale
columns. Moreover, each production batch has
undergone extensive quality control testing to ensure
reliable lot-to-lot performance.
The availability of a wide range of pore sizes, with
narrow distribution, permits the utilization for a broad
range of applications allowing the optimization of
selectivity and capacity. Tight particle size distributions
are ideally suited to choose the best compromise
between efficiency, operating pressure and process
time, thus offering the best balance between price and
performance.
CHROMATOREX-SMB spherical silica gels are
manufactured to very high chemical purity standards.
Organics and trace metals content are controlled within
stringent limits. Reproducible separation results,
allowing direct scale-up without time-consuming
methods redevelopment, are the direct result of the
rigid control of chemical purity, surface area, pore
volume, and pore size.
Featuresss & Benefits
◆ Selection of particle size to optimize efficiency.
◆ Narrow particle size distribution to reduce pressure.
◆ Consistent and reproducible retention times to allow
direct scale-up from lab through process applications.
◆ Lot-to-lot consistency for reproducible performance.
◆ Bulk quantities for large-scale applications, no scale-up
limitation, secured lots offered for process use.
Characteristics
SMB70 | SMB100 | SMB200 | SMB300 | ||
---|---|---|---|---|---|
Nominal Pore Diameter | nm | 7 | 10 | 20 | 30 |
Specific Surface Area | m2/g | 480 | 270 | 130 | 90 |
Particle Sizes | オm | 5, 10, 15, 20, 20/45 | |||
Specific Pore Volume | ml/g | 0.8 | |||
pH (5% suspension) | – | 5.5 | |||
Density | g/ml | 0.4 | |||
Water Content | % | <2 | |||
Chemical Analysis | SiO2 % | 99.9 | |||
Na2O % | 0.02 | ||||
Fe2O3 % | 0.01 | ||||
CaO % | 0.02 | ||||
Al2O3% | 0.03 |
Particle Sizes
For analytical HPLC, CHROMATOREX-SMB offer a
selection between 3 and 5 オm. The 5 オm particles are
particularly recommended for method development
start-up to help the optimization of the method.
For preparative HPLC, either at pilot stage or for high
value purification processes, the 10 オm particles are
preferred for those applications requiring higher
resolution, while the 15 オm particles offer a good
compromise between performance and cost.
The larger particle size 20 and 20 / 45 オm are well
suited for medium pressure columns.
Pore Sizes
CHROMATOREX-SMB with pore sizes 7, 10 and 20
nm are recommended for the purification of natural
and synthetic small molecules, lipid soluble organic,
small organic chemical compounds.
Larger pore sizes, 30, 50, 80 or 100 nm are
preferred to optimize the capacity and the yield for
the purification of medium to large peptides and
proteins.
Spherical Silica / MB
Description
CHROMATOREX-MB, Micro Bead spherical silica
gels, manufactured by Fuji Silysia, are ideally suited
for the normal phase chromatography, flash
chromatography and absorption.
ALL CHROMATOREX-MB spherical silica gels are
mechanically robust. Therefore, they can be repacked
multiple times in either analytical or preparative scale
columns. Moreover, each production batch has
undergone extensive quality control testing to ensure
reliable lot-to-lot performance.
The availability of a wide range of pore sizes, with
narrow distribution, permits the utilization for a broad
range of applications allowing the optimization of
selectivity and capacity. Tight particle size distributions
are ideally suited to choose the best compromise
between efficiency, operating pressure and process
time, thus offering the best balance between price and
performance.
CHROMATOREX-MB spherical silica gels are
manufactured to very high chemical purity standards.
Organics and trace metals content are controlled within
stringent limits. Reproducible separation results,
allowing direct scale-up without time-consuming
methods redevelopment, are the direct result of the
rigid control of chemical purity, surface area, pore
volume, and pore size.
Featuresss & Benefits
◆ Selection of particle size to optimize efficiency.
◆ Spherical particle shape to reduce pressure.
◆ Consistent and reproducible retention times to allow
direct scale-up from laboratory through process
applications.
◆ Lot-to-lot consistency for reproducible performance.
◆ Bulk quantities for large-scale applications, no scale-up
limitation, secured lots offered for process use.
Characteristics
SMB70 | SMB100 | SMB300 | ||
---|---|---|---|---|
Nominal Pore Diameter | nm | 7 | 10 | 30 |
Specific Surface Area | m2/g | 480 | 270 | 90 |
Particle Size Distribution | オm | 40/75 & 75/200 | ||
Specific Pore Volume | ml/g | 0.8 | ||
pH (5% suspension) | – | 7 | ||
Density | g/ml | 0.5 | ||
Water Content | % | 5 | ||
Chemical Analysis | SiO2 % | 99.9 | ||
Na2O % | 0.02 | |||
Fe2O3 % | 0.01 | |||
CaO % | 0.02 | |||
Al2O3% | 0.03 |
Particle Sizes
Silica gel quality is critical to achieve optimum
performance when using the Flash chromatography
method.
The particle size distribution and the particle shape
determine the resolution and the speed.
For efficient mass transfer, CHROMATOREX-MB
spherical silica gels have a tight control of particle
size distribution allowing less than 10% particles
upside and downside the defined distribution range.
Two standard particle size distributions are offered.
Pore Sizes
Surface area, water content and surface pH
determine the capacity of the silica gel.
CHROMATOREX-MB is fully porous silica, and the
rigorous control of the pore volume and pore size
distribution ensures the accessibility for the targeted
molecule to reach the maximum surface area.
CHROMATOREX-MB with pore sizes 7 and 10 nm
and recommended for the purification of natural and
synthetic small molecules, lipid soluble organic,
small organic chemical compounds.
Larger pore sizes, 30 nm are preferred to optimize
the capacity and the yield for the purification of
medium to large peptides and proteins.
Granular Silica
Description
CHROMATOREX-GS, granular silica gels,
manufactured by Fuji Silysia, are the most popular
grade for the normal phase adsorption chromatography.
Mechanically robust, CHROMATOREX-GS granular
silica gels are cost effective, and therefore they are
very often used only one time, and not regenerated,
due to the cost of the solvent regeneration versus the
price of the silica.
Fuji Silysia is certainly the sole company, due to its
historical roots, to manufacture the base material for
the production of the silica gels. By doing this, we are
ensuring a high level of consistency and reproducibility.
In addition our large scale production, in multi 1,000
tons, of silica gels per year, give us a significant
advantage in the process control, resulting in
outstanding quality and reliable lot-to-lot performance.
CHROMATOREX-GS granular silica gels are
manufactured to very high chemical purity standards.
Organics and trace metals content are controlled within
stringent limits. Reproducible separation results,
allowing direct scale-up without time-consuming
methods redevelopment, are the direct result of the
rigid control of chemical purity, surface area, pore
volume, and pore size.
Featuresss & Benefits
◆ Selection of particle size to optimize efficiency.
◆ Large surface area to optimize the capacity.
◆ Consistent and reproducible retention times to allow
direct scale-up from laboratory through process
applications.
◆ Lot-to-lot consistency to assure reproducible
performance.
◆ Bulk quantities for large-scale applications, no scale-up
limitation, secured lots offered for process use.
Characteristics
SMB60 | ||
---|---|---|
Nominal Pore Diameter | nm | 6 |
Specific Surface Area | m2/g | 500 |
Particle Sizes | オm | 20/45, 40/75 & 75/200 |
Specific Pore Volume | ml/g | 0.75 |
pH (5% suspension) | – | 6.5 |
Density | g/ml | 0.5 |
Water Content | % | 5 |
Chemical Analysis | SiO2 % | 99.9 |
Na2O % | 0.02 | |
Fe2O3 % | 0.01 | |
CaO % | 0.02 | |
Al2O3% | 0.03 |
Particle Sizes
CHROMATOREX-GS are particularly well adapted for
the application not requiring a high number of
theoretical plate, such as open column, or low
pressure column, or even batch process.
For efficient mass transfer, CHROMATOREX-GS have
a tight control of particle size distribution allowing less
than 10% particles upside and downside the defined
distribution range.
Three standard particle size distributions are offered:
◆ 30 microns (20/45)
◆ 50 microns (40/75)
◆ 150 microns (75/200)
Special grades for industrial applications are offered in
large bulk quantities:
◆ 30 / 50 microns
◆ 53 / 250 microns
◆ 45 / 100 microns
◆ 75 / 150 microns
◆ 75 / 200 microns
Pore Sizes
CHROMATOREX-GS with 6nm pore size and a
surface area of 500 m2/g is recommended for the
purification of natural and synthetic small molecules,
lipid soluble organic, small organic chemical
compounds.
The large capacity of the CHROMATOREX-GS makes
it widely use for crude pre-purification step.
Bonded Silica
Description
All our silica gels CHROMATOREX can be derivatized
to produce a bonded stationary phase for reverse
phase chromatography or some specific
chromatography types.
Derivatization
The growing interest in complex synthetic or
recombinant peptides has expanded the need for high
performance bonded phase process chromatography.
The use of bonded phases for injectable biological
products has also created new demands.
Optimized quality:
The fact that we manufacture the starting gels in very
large batch quantities constitues an important
contribution to the overall reproducibility of these
reversed phase silica.
Moreover, our production capacity for derivatization
per batch contributes also to a high level of
reproducibility.
Each lot is extensively tasted for carbon load and
chromatography retention times, efficiency and peak
symmetry. The quality of the surface coverage is
tasted by the Phenol/Pyridine test to evaluate any
remaining free acid groups on the silica surface.
Optimized chemistry:
Chromatorex silica gels are manufactured with mono-
functional organo-silanes, and finished with an end
capping surface treatment.
On request, tri-functional, with or without end capping
treatment could be performed.
Each lot is extensively tasted for carbon load and
chromatographic retention times, efficiency and peak
symmetry. The quality of the surface coverage is
tasted by the Phenol/Pyridine test to evaluate any
remaining free acid OH groups on the silica surface.
Product Range
C18 Chromatorex:
This is the most versatile and popular reversed phase.
The choice of the pore size must be carefully selected
in relation to the molecular weight of the molecule.
C8 Chromatorex:
Universal, but less performing than C18, it is the
primary choice for investigation.
Phenyl Chromatorex:
Specific reversed phase for medium / polar molecules,
shows a high selectivity for acceptor / donor
compounds.
C4 Chromatorex:
Extremely performing when bonded in wide pore silica
for bio-chromatography and polar compounds.
NH2 Chromatorex:
The Amino-silica gels will separate a variety of sugars
with acetonitrile / water mobile phase. Alternatively
with hexane / ethyl acetate mobile phase, it will
function as a ‘less active’ normal phase.
In addition, since the surface contains primary amino
groups, NH2 Chromatorex can be used as a base for
coupling chiral ligands.
CN Chromatorex:
Can be used in both normal and reversed phase
chromatographies, for steroids, catechols, …
Diol Chromatorex:
Particularly used for aqueous GPC applications, Diol
Chromatorex could be also used for normal phase
chromatography mode for the separation of phenols for
example.